유세포분석실
The Flow Cytometry Core Facility is located on the second basement
(Room B2 306) in Seoul National University Hospital, Center for
Medical Innovation (CMI) building. The flow cytometry works at
Biomedical Research Institute are divided into two broad categories,
analysis and cell sorting. The facilities are extremely well equipped
for both analysis and cell sorting. A brief description of the
cytometers and their particular capabilities is as follows. Please
contact our staffs for more information.
FACSCalibur(Becton&Dickinson) : 3-color, 1-laser
FACSCalibur(Becton&Dickinson) : 4-color, 2-laser
FACSCanto(Becton&Dickinson) : 10-color, 3-laser
FACSAria I (Becton&Dickinson) : 3-laser (488nm, 640nm, 355nm )
FACSAria II (Becton&Dickinson) : 3-laser (488nm, 640nm, 355nm )
BSC-II system
All of the flow cytometers (
FACSCalibur, FACSAria etc.) are solely operated by well trained staffs
with the exception of some analytical experiments done by experienced
users who completed user education. To make the reservation, please
contact us at 2072-1852.
Flow cytometry, typically using fluorescent probes which bind to specific cell associated molecules, allows measurements of various phenotypic, biochemical and molecular characteristics of individual cells (or particles) suspended in a fluid stream. As the cells flow past a focused laser beam of appropriate wavelength, the probes fluoresce and the emitted light is collected and directed to appropriate detectors. These detectors, in turn, translate these light signals into electronic signals proportional to the amount of light collected. Information regarding the relative size and granularity of a cell, for example, is also obtained as these characteristics influence the way in which light is scattered as the cell passes through the laser beam.
For more informationLocation: Room B2 306, 2nd basement, Center for Medical Innovation (CMI)
Person in charge : Ji Hyun Sung, Mira Song
Phone: 02-2072-1852 Fax: 02-762-5178